• M30-Apoptosense is an enzyme-linked immunosorbent assay (ELISA) CE-marked for the quantitative detection of caspase-cleaved Keratin-18 (ccK18, K18F, K18Asp396-NE: M30 neo-epitope) levels in cytosolic cell extracts, cell culture supernatants, human serum or plasma or other body fluids.
  • The M65 EpiDeath® ELISA is the next generation of Peviva's biomarkers. It is intended for the quantitative detection of keratin 18 (K18) present in cell culture supernatants, human serum, human plasma or other body fluids after it has been released from dying cells into the extracellular compartment.
  • The M30 CytoDeath™ ELISA has been specificallydeveloped for cell culture applications. The CDE assay has a higher dynamic range suitable for in vitro work making it a powerful drug screening tool and is useful for in vitro characterisation of apoptosis-inducing drugs.
  • Apoptosis induced by either death-inducing receptors or other stimuli leads to activation of specific caspases. Subsequently, apoptotic cells are eliminated by an intrinsic suicide program, resulting in DNA fragmentation, nuclear condensation, cytoskeletal reorganization, plasma membrane blebbing and loss of cell adhesion.
  • The epithelial keratins (IF types I and II, also called cytokeratins) are conserved phylogenetically and are closely related, biochemically and imunologically. Keratins 1-8 constitute the type II group (53-68 kDa, neutral to basic protein components), while keratins 9-20 constitute the type I group (40-56 kDa, acidic proteins).
  • Lancet Gastroenterol Hepatol. 2018 ;3:104-113. Risk stratification after paracetamol overdose using mechanistic biomarkers: results from two prospective cohort studies. Dear JW, Clarke JI, Francis B, Allen L, Wraight J, Shen J, Dargan PI, Wood D, Cooper J, Thomas SHL, Jorgensen AL, Pirmohamed M, Park BK, Antoine DJ. Lancet Full Text Supplementary Information
  • Product Fact Sheets, Product Manuals (Instructions for Use), MSDS
  • BIOAXXESS acts as the distributor of VLVbio, Sweden for customers in the UK and the Republic of Ireland since 2011. Our scientific and technical experience for PEVIVA's M30 and M65 ELISA and Cytokeratin-18 antibodies stems back to 2003 when BIOAXXESS started trading as a technology and business development agency for PEVIVA in Europe. Our PhD-level expert technical support and indepth scientific insight are offered to our customers at universities, hospitals, CROs, clinical research centres and R&D within pharma and biotech in the UK and ROI. BIOAXXESS office and distribution is based in the West Midlands, Tewkesbury and we operate a next day delivery policy.

Featured products

  • M30 Apoptosense® ELISA #10011

    M30 Apoptosense® ELISA #10011

    £495.00
    M30 Apoptosense® ELISA (ccK18, K18F, K18Asp396NE-M30) M30 Apoptosense® is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of caspase-cleaved Keratin-18 (ccK18, K18F, K18Asp396-NE: M30 neo-epitope) levels in cytosolic cell extracts, cell culture supernatants, human serum or plasma or other body fluids.
  • M65® ELISA (classic)

    M65® ELISA (classic)

    £495.00
    The M65® ELISA is capable of measuring the amount of Keratin 18 that is released from the dying cells of many cell cultures that are from the extracellular compartment. The M65® ELISA offers the opportunity to quantify total cell death of K18 positive cells.
  • M30 CytoDeath™ ELISA Research Use Only

    M30 CytoDeath™ ELISA Research Use Only

    £175.00
    The M30 CytoDeath™ ELISA is a unique product specifically for cell culture applications. This is a powerful drug screening tool and is useful for in vitro characterisation of apoptosis-inducing drugs. It also offer the ability to quantify apoptosis in multicellular spheroids and organ culture systems.
  • M65 EpiDeath® ELISA

    M65 EpiDeath® ELISA

    £495.00
    The M65 EpiDeath® ELISA is the next generation of Peviva's biomarkers and is a modified version of the M65® ELISA and is intended for the samples with high Keratin 18 plasma and serum levels (e.g. sepsis, GvHD and liver toxicity such as DILI or ALF).

Welcome to BIOAXXESS PEVIVA e-Shop!

PEVIVA Product Application Guide

Measure apoptotic epithelial cell/tissue death (only Apoptosis) in human blood samples or in cell/tissue culture (3D liver cell or multicellular tumour spheroids (MTS) lysates: M30 Apoptosense® ELISA   Measure total epithelial cell /tissue death (Apoptosis plus Necrosis) in human blood samples or in human cell/tissue culture supernatants: M65® ELISA (Classic)  and for DILI M65 EpiDeath® ELISA  

  • Detect epithelial (carcinoma) tissue Keratin 18 in FFPE sections: M6 or M5 K18 MAbs 
  • Measurement of apoptotic epithelial cell/tissue death in FFPE sections, Western Blot, or FACS using different fluorochromes or Biotin as conjugate:M30 CytoDEATHTM MAb 
  • NEW: Measurement of tumor cell/hepatocyte apoptosis in vitro using 3D liver cell or multicellular tumour spheroids (MTS) lysates (cancer cells/hepatocytes: K18-positive) on their own or in co-culture with K18 negative endothelial cells, fibroblasts (stroma) or infiltrating immune cells: M30 CytoDeathTM ELISA in combination with the novel Cytoskeleton Solubilisation Buffer (CSB, please enquire).
  • Measurement of apoptotic epithelial cell/tissue death in human cell/tissue culture lysates: M30 CytoDeathTM ELISA

  •