M5 and M6 Cytokeratin 18 Monoclonal Antibodies (K18) Research Use Only Cat. No. #10600-10650
M5 / M6 Keratin 18 (Cytokeratin 18) monoclonal antibodies (FFPE-grade) (Prod. No. 10600-10650)
In eukaryotic cells, the cytoskeleton is composed of three different types of morphologically distinct filamentous structures:
- intermediate filaments (IF)
The epithelial keratins (IF types I and II, also called cytokeratins) are conserved phylogenetically and are closely related, biochemically and immunologically. Keratins 1-8 constitute the type II group (53-68 kDa, neutral to basic protein components), while keratins 9-20 constitute the type I group (40-56 kDa, acidic proteins).
Upon release from dying cells, keratins provide useful markers for epithelial malignancies, distinctly reflecting ongoing cellular turnover. Certain keratins, the most prominent example being keratin 18, are substrates for lethal caspase activation and the subsequent release of defined protein fragments occurs during apoptotic cell death.
The clinical value of determining different soluble keratin protein fragments in blood samples (i.e. using the M30 Apoptosense® ELISA or M65® ELISA) lies in the rapid and non-invasive assessment of drug efficacy or response to therapy in epithelial cell carcinomas.